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An aliquot of 20 l of the third-round virus mixture was used to infect 500 ml (SFM900-II medium with 5% FBS, Thermo Fisher) of Sf9 cells (1.5 10 6 /ml) for protein expression in an orbital shaker (Shanghai Zhichu Instrument) at 27 C and 200 rpm

Among the tested matrices, M05 and M09 demonstrated better retention of viable cells during the later fermentation phase, as supported by both DeltaLog (CFUs) values trends

If you have already used or are currently using either of these compounds, it is important to disclose this to your GP or pharmacist without delay